Three-dimensional 3D cell models offer enormous innovation potentials for instance in medicine, to treat patients in a highly individualised way or in the food industry for the development of alternative methods to produce clean meat.

However, the assessment and long term monitoring of these complex 3D tissue analogues need versatile imaging techniques that can capture not only morphological, but also functional and molecular information for omics analysis, namely transcriptomics, proteomics and metabolomics. This remains a challenge for conventional analytical methods. Current imaging methods offer strengths but have also weaknesses according to their techniques. In this workshop we want to discuss approaches to progress in the non-destructive investigation of advanced 3D cell models with spatially resolved imaging technologies.